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Following immunostaining, L3 central nervous system (CNS) or body walls were mounted in SlowFade Gold (Invitorgen) and fluorescent images were acquired on a LSM 980 (Carl Zeiss) using a 20 objective (for the CNS) or 40/63 objective (for the NMJ)

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Interestingly, many of the hub targets have been reported to be involved in cell proliferation, tumorigenicity, and other biological functions, and we will explore these further in the following discussion section

Glutathione injections are administered intravenously (IV) to lighten skin by reducing melanin production