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To determine possible mechanisms, we used iTRAQ labeling to identify proteins that were differentially expressed between the control and H 2 O 2 -treated zygotes

The calculation procedure can be found in Additional file 1: Fig S2

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Role of inflammatory mediators in AILI Cytokines, which have various functions including immune regulation, intercellular signal transmission, and damaged tissue repair, etc., are small molecule proteins produced by immune cells and some non-immune cells

This is standard injection technique covered in the GLP-1 injection guide