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body weights and body composition were assessed throughout the study (Fig

Additionally, deubiquitination impacts the localization and activity of proteins within the cell, altering interactions with other molecules and thus regulating protein function [119]

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qPCR (20 L) was carried out by using 2 Ultra SYBR Mixture (CWbio, China) in 12 8 well plates in a CFX Connect Real-Time System (Bio-Rad), containing 10 L of SYBR Mixture, 2 L cDNA, 1.6 L of qPCR primers (4 M), and 7.4 L nuclease-free water

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10.1038/nrcardio.2017.157 129