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Briefly, hBMECs were sub-cultured onto polyester 12-well transwell inserts with a 0.4 m pore size (Corning) coated with 1:1 collagen IV (100 g mL 1 , Sigma) and fibronectin (50 g mL 1 , Sigma) at a density of 1 10 6 cells mL 1 and allowed to reach confluence for another two days