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Nonspecific bound proteins were washed out with buffer A supplemented with 25 mM imidazole (Thermo Scientific, A10221) and laccases were eluted with buffer A containing 250 mM imidazole

If the provided concentrations of the actives are high enough, and the topical product is repeatedly applied as a leave-on product, like in the current study, we believe that sufficient amounts of actives reach the hair bulb

Med Sci (Basel)

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Costs $15 to $25 for 30ml which reconstitutes dozens of vials