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Histopathological analysis involved fixing left lung lobes in 10% neutral formalin, embedding in paraffin, sectioning into 4 m thick sections, and staining with hematoxylin and eosin (H&E)

10.1016/j.jpsychires.2020.02.023 12 CabreraR

Briefly, recombinant PDI protein (0.4 M) was incubated with indicated compounds at 37 C for 1 h in sodium phosphate buffer (100 mM sodium phosphate, 2 mM EDTA and 8 M DTT, pH 7.0)

By supporting connective tissue health and overall well-being, BPC 157 could help enhance quality of life in senior pups