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This was positioned prior to the takeoff of the obtuse marginal branch of the LCx to create consistency across animals in ischemic territory generation and gently retracted for 2 minutes

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Membranes were incubated overnight at 4 C with Cleaved Caspase-3 (Asp175) (Cell Signalling Technology) and with peroxidase-linked anti-rabbit IgG or anti-mouse IgG secondary antibodies (GE Healthcare Life science) for 1 h at room temperature

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"We don't know if there's a benefit, or if there's increased risk, if you get to pregnancy at a lower weight and then come off the medication," said study first author Dr