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(control- sample)]) were normalized to the house keeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and used as a reference

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Impaired homocysteine metabolism-mediated moderate hyperhomocysteinemia (caused by folate deficiency, vitamin B 6 deficiency, or by methionine excess) might thus have primarily pro-oxidant effects resulting from an altered extra- and intracellular redox potential that can affect essential vascular functions

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