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The tGSH concentration was quantified using the enzymatic recycling method in which rates of 5-thio-2-nitrobenzoic acid (TNB) formation were calculated after the addition of the DTNB (1.8 mM) solution (Ellmans reagent) and glutathione reductase (250 units per ml) solution to the samples
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In this study, it was also demonstrated that treatment with the inhibitor of cholesterol synthesis fluvastatin (Fig
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