Cells were exposed for 48 h, the cleared cell extracts were incubated with DEVD-p-nitroanilide and caspase-3 activity was measured spectrophotometrically
As sulfur amino acid input rises, so does glutathione concentration but not as fast as it would if the inhibition were not present
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Briefly, 100 L of CuCl 2 (1 M, 13.445 mg) solution was added to 10 mL of BSA (10 g L 1 ) solution and stirred at room temperature (RT, 25 C) for 5 min