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Nucl Acid Res 1986
The sections were then washed and incubated with biotinylated goat anti-rabbit immunoglobulin (1100) for 3 h at room temperature, washed with 0.1 M PBS (pH 7.4) and incubated with avidin-biotin complex tagged with horse redox peroxidase enzyme prior to development with 3, 3-diaminobenzidine (ABC kit, SantaCruz)
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