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Purification of microsomal glutathione S-transferase [PMID: 7080784] Laboratory literature summary: Purification of microsomal glutathione S-transferase
Western blotting Frozen cortex or hippocampal samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail
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In contrast to the structural function played by the fibrous proteins, the globular proteins are chemically reactive, serving as enzymes (catalysts), transport agents and regulatory messengers
Booth, S
