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These processes that involve oxygen result in waste products called free radicals
Archived from the original on 8 November 2022
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The relative activity of the apo-GT enzyme was restored by about 8588% in response to Zn 2+ , Cu 2+ , and Fe 3+ , that is partially consistent with the glutathione dependency as cofactor of GT of Pseudomonas putida [3]
50 mM iodoacetic acid was added to prevent the formation of disulfide bonds of reduced cysteine residues, and samples were stored at 80C
