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Prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), 20 g of frozen, ground, and fresh tissue was homogenized in 20 mL of extraction buffer containing 100 mmol L 1 TrisHCl (pH 6.8), 10 g L 1 SDS, 10 mmol L 1 dithiothreitol (DTT), 1 mmol L 1 ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), and a protease inhibitor mixture (cat

Of the 4 microbes, 1 had not been reported at the time of isolation, but showed 99% 16S-rRNA gene sequence identity (Supplemental Figure 4B) with an organism recently isolated by others and classified as Emergencia timonensis (37)

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