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Oxidative stress in childhood obesity
Oxidized redox state of glutathione in the endoplasmic reticulum
Then a biotinylated detection antibody specific for Mouse GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
doi:10.1017/S0022215112000382
Slices were incubated in 32C oxygenated artificial cerebrospinal fluid (in mM: 125 NaCl, 2.5 KCl, 2 CaCl 2 , 1.3 MgCl 2 , 1.3NaH 2 PO 4 , 1.3 Na-ascorbate, 0.6 Na-pyruvate, 25 glucose, and 25 NaHCO 3 ) for at least 1 hour before recording
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