For a common working concentration of 2.5 mg/mL, add 2 mL of solvent directly to the lyophilized vial and allow it to dissolve gently without vortexing, as aggressive agitation can cause peptide aggregation and loss of bioactivity
During the transient ischemia and the period of reperfusion many cells generate byproducts of oxygen that are toxic to the heart
You are now getting $170 to $190 worth of medication from your $200 vial
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Indeed, it is known that the enzyme -glutamyltranspeptidase (GGT) on the external cell membrane initiates this process (called the -glutamyl cycle) [12, 50, 51]