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Higher incidence of anemia Complications in Pregnancy Due To Neural Tube Defects, posterior Tongue tie, lip tie Anencephaly Other neural tube defects Spina bifida Autism Rheumatoid Arthritis Flares Down Syndrome PEs Altered drug metabolism Low vitamin d levels/ osteoporosis Neuropsychiatric Disorders Gestational Diabetes Early Pregnancy Loss/ SAB (viable fetus) Placental Abruption, Low Birth Weight The most common MTHFR gene mutations are found at position 677 and/or position 1298 on the MTHFR gene

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The following reagents were procured from Beyotime Institute of Biotechnology (Shanghai, China): mitochondrion-selective probe (Mito-Tracker Green), DCFH-DA, mitochondrial membrane potential assay kit (JC-1), Annexin V-FITC/propidium iodide (PI) apoptosis and necrosis detection kit, H&E staining kit, and One Step TUNEL Apoptosis Assay Kit