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To ensure that the resulting increased permeabilization was entirely due to TGF 1 signal transduction, monolayers were treated with rTGF 1 in combination with a TGF RII antagonist, GW788388

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Matrix Biol 17:6573 Halper J (2014) Advances in the use of growth factors for treatment of disorders of soft tissues

Many protocols apply AHK-Cu first, allow 15 to 20 minutes for absorption, then apply minoxidil

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