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& Malankiewicz, A

Metabolism 78:4351

Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

Have trouble burning fat, even with diet and exercise

Quantitative and digital PCR Quantitative PCR was performed on purified genomic DNA using TaqMan Advanced Master Mix (Applied Biosystems), 250 nM primers and 333 nM probes (sequences listed in Supplementary Dataset 1), and a QuantStudio6 (Applied Biosystems)

Malva parviflora Extract (5FAD) reduced microglial pro-inflammatory MI phenotype, promoted phagocytosis via CD36 upregulation, and reduced amyloid plaques