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The analyte and internal standard were extracted from 50 l of plasma by solid phase extraction

Amino acids are the fundamental components of proteins and are necessary in many internal functions of the body

Lv M, Zhu X, Wang H et al (2014) Roles of caloric restriction, ketogenic diet and intermittent fasting during initiation, progression and metastasis of cancer in animal models: a systematic review and meta-analysis

Lifestyle factors such as hydration and exercise can also influence the duration of benefits

Rare side effects: itching, exanthema, chills, fever, hot flushes, nausea, dizziness, and very rarely anaphylaxis

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIF