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Cells Preparation and treatment Mouse distal convoluted tubule cells (MDCTs) were purchased from ATCC (ATCC Cat# CRL-3250, RRID: CVCL_CZ90) and maintained in DMEM supplemented with 5% fetal bovine serum (FBS), 1% penicillin-streptomycin in a humidified incubator at 37 C with 5% CO2
A pivotal 2021 clinical study of more than 1,900 adults, known as the STEP 1 trial, laid the groundwork for the use of these drugs as a treatment for weight loss
The ingredient also contributes to immune-support positioning through its polysaccharide content, allowing it to pair effectively with broader mushroom wellness systems
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