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Agar gels were diluted with 1 mL PBS, melted at 85 C for 3 min, and centrifuged at 40 C (5 min at 1800 g ) to pellet the cells

10.1007/s00109-023-02308-5 24 MalacridaS.GiannellaA.CeolottoG.ReggianiC.VezzoliA.Mrakic-SpostaS.et al (2019)

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Improperly stored or handled peptides can degrade before use, reducing efficacy or, in some cases, producing unintended effects

To confirm that the observed phenotype was solely caused by the connection between the striatum and the SNcVTA region, we used the periaqueductal gray (PAG) region as an internal control