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Gene expression of inflammatory markers associated with leucocyte recruitment, adhesion, and migration in atherosclerotic aortas was evaluated

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RT-PCR reactions were performed in a total volume of 25 l containing 25100 ng of total RNA, 200 m of each dNTP, 1.5 m MgCl 2 , 25 pmol of each primer, 2.5 U of AMV RT and 2.5 U of TFL DNA polymerase (Access RT-PCR System, Promega)

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