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Short nights raise ghrelin and blunt the satiety hormones the entire protocol is built around, so a poorly slept week can blunt the appetite suppression you paid for

The effects of selenium and copper deficiencies on glutathione S-transferase and [PMID: 3435464] Laboratory literature summary: The effects of selenium and copper deficiencies on glutathione S-transferase and glutathione peroxidase in rat liver

Targeting Glutamine Transporters Inhibiting transporters like SLC1A5 cuts off the glutamine supply, further restricting growth

Each component is tested at ~99% HPLC purity with a COA

(B6, especially in the pyridoxine form, is one of the few B vitamins that can lead to toxicity when used in excess.) Threonine is also important to gut barrier integrity in the small intestine, and I have read some reports of it helping with minimizing reactions to gluten, although I have not studied this in great depth yet

Zhao, J