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As positive controls, PSCs were pre-incubated with Rosup reagent (Beyotime) for 20 min
The optical density was measured spectrophotometrically at 564 nm with an ELIZA microplate reader (Meter tech
They can support mechanistic hypotheses about endothelial response and microvascular repair, but they do not establish human wound healing efficacy, cardiovascular benefit, clinical safety, or suitability for any condition where abnormal angiogenesis could be biologically relevant
For rib injuries specifically, the stack addresses the full scope of tissue damage
Cell 156(12):317331
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