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Single-label FISH for Glp1r or Gcg Spinal cord cryosections collected at 1 mm interval were hybridized with digoxigenin-labeled riboprobes for Glp1r (corresponding to 11-1402 of the mouse Glp1r mRNA, Genbank # NM_021332.2) 60 or Gcg (corresponding to 75-944 of the mouse Gcg mRNA, NM_008100.4)

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2.5 Protein synthesis assay The surface sensing of translation (SUnSET) method (Schmidt et al., 2009) was used for protein synthesis assay

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Meanwhile, glutathione, one of natures most potent antioxidants, neutralizes free radicals and imparts an inner glow to the skin, while protecting it from premature aging

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