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Crystals of HT adducts formed in a couple of days in 25% vol/vol PEG smear medium and 0.1 M Tris pH 8.5 (MeTRaQ-G), in 20% vol/vol PEG 6000, 10% ethylene glycol, 0.1 M magnesium chloride hexahydrate and 0.1 M MES pH 6 (TRaQ-G) and in 20% vol/vol PEG 6000, 0.1 M lithium chloride and 0.1 M sodium citrate pH 4 (TRaQ-G-ctrl)

The hulls (the hard outer shells of popcorn kernels) can be particularly difficult to digest and may cause irritation in sensitive individuals

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