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Ballard C, Nrgaard CH, Friedrich S, Mrch LS, Gerds T, Mller DV, et al

Vitamin D is a fat-soluble vitamin and essential nutrient that supports calcium absorption, bone health, immune function, and overall wellness

Purifications were conducted by preparative RP-HPLC, eluting with a linear gradient (20 ml min 1 ) and using a binary solvent system consisting of H 2 O:MeCN:TFA (buffer A, 95:5:0.1

Cells were extracted with 200 L of ice-cold 80% methanol, centrifuged at 15,000 g for 20 min at 4C and lyophilized

The relevant federal statute is the Federal Food, Drug, and Cosmetic Act