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These are laboratory research observations only, not established effects in humans
Membranes were washed 4 times in PBS-T, followed by addition of secondary antibody (dissolved in 5 % milk in PBS-T) for 1 h purchased from LI-COR containing a fluorophore (IRDye 800CW/680RD goat anti-mouse or goat anti-rabbit) for detection by the Odyssey DLx Imaging system
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Human Fos-related antigen 1 (FOSL1) and Heat Shock 70 kDa Protein 1A (HSPA1A) ELISA kits were purchased prom MyBioSource (San Diego, CA, USA)
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