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Enrollment and Randomization: If all eligibility criteria are met and the individual decides to join the trial, they are enrolled and may be randomly given either the investigational medication or a comparison treatment, depending on the study design

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Because tirzepatide also acts on the GLP-1 pathway, it slows gastric emptying in much the same way, so the gut side effects tend to look familiar

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The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)