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Protein samples (20 g) were separated on NuPage Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes
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Obesity is recognized by major medical organizations as a chronic, relapsing disease, which means care decisions should be individualized and approached with a long term mindset rather than treated as a short term fix
Acta Pharmacol Sin 2000
After addition of loading buffer, lysates were vortexed, and supernatants were heated to 95 C for 5 min
B12 deficiency associates with depression, irritability, and mood disturbances
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