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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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A later congressional investigation described an unusually close collaboration between FDA staff and Biogen during the review.In 2022, an FDA panel rejected the ALS drug Relyvrio for weak data, then reversed course after ALS patients lobbied hard at a second meeting