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The quality of the oocytes used in this study was selected based on homogenous cytoplasm and compact cumulus cells, ensuring they had similar developmental potential, and as a result, the oocyte morphology after thawing was expected to be consistent

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Cells were cultured for 2 h and then read using a ClarioStar-Plus plate reader at absorbances of 570 and 595 nm

doi: 10.3389/fimmu.2022.853015 156 LaforgeM.ElbimC.FrreC.HmadiM.MassaudC.NussP.et al