This study included 12 week-old Wistar albino male rats that were bred in-house at the animal facility at the Department of Pharmacology, School of Medicine in Zagreb, Croatia
A report from the Framingham Offspring Study of 2,999 adults found the following average daily B12 intakes, including fortified foods and supplements (Tucker, 2000): Age 26-49: 8.9 g Age 50-64: 8.7 g Age 65-83: 8.5 g A 2015-2016 National Health and Nutrition Examination Survey of over 4,500 adults 60 years and older found the following average daily B12 intakes (USDA NHANES, 2019): Male non-supplement users (68% of men): 4.5 g Male supplement users: 5.3 g from food plus 326 g from supplements Female non-supplement users (63% of women): 3.6 g Female supplement users: 3.8 g from food plus 520 g from supplements European Food Safety Authoritys Recommendations In 2015, the EFSA released recommendations for B12 intake across Europe
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This may be beneficial for individuals with severe deficiencies or specific health conditions that require higher levels of supplementation
Some medical conditions like individuals with gastrointestinal disorders such as Crohns disease, and celiac disease, or those who have undergone gastric bypass surgery may also be at risk of developing vitamin B12 deficiency due to impaired absorption