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When converting a fill volume into molarity, use 403.9 g/mol for the complex, not 340.4 g/mol for the free tripeptide
Holotoxin delivery and cell viability (CellTiter-Glo assay) 6His-tagged proteases fused to the BoNT translocation domain via a thrombin-cleavable linker were expressed and purified as described above
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This form encapsulates glutathione molecules within tiny phospholipid bubbles, essentially creating microscopic protective spheres that can survive the harsh acidic environment of the stomach and pass through intestinal walls intact
L.LaversanneM.SoerjomataramI.JemalA.et al (2021)
Expert Opin Ther Targets (2015) 19:144764
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