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GLP-1MK-801 was incubated in PBS with 200 mM glutathione at pH 7.0
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Notice that GSH goes up for low estradiol values, reaches a peak and descends for high estradiol values Variation of GSH steady state depending on S In our model, we take the activation of GCL and GPX by estradiol to be 80%, which is a reasonable choice supported by the experimental and clinical data discussed above
Concentrations typically used in cell culture range from 10 ng/mL for sensitive signaling assays to 100 ng/mL for full receptor saturation studies
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