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Treatment of the proteins with DTT prior to application to the array allowed us to distinguish between those proteins where BioGEE had been incorporated into the protein via a non-disulphide mechanism (possibly due to interaction with biotin) from those that formed disulphide bonds with the GSH in BioGEE and hence were more likely to represent potential targets of glutathionylation
There are biological limits to what any one therapy can accomplish. Instead, it may reflect the limit of what this type of treatment can achieve for a given patient
Notice the limitation immediately
1997 Jan;46(1):89-96
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