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For the generation of mRFP-zDHHC5-KASH wild-type and DHHS-mutant, the sequences mRFP-zDHHC5 and mRFP-zDHHS5 were PCR-amplified from mRFP-fusions, cut with NheI/KpnI and used to exchange the GFP sequence cut with the same enzymes from GFP-KASH

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This varies, given the color of your skin, the dose applied, and how you respond to treatment

Pernicious anemia is a chronic condition characterized by the inability to absorb vitamin B12, leading to deficiency and related complications. Other issues like problems after stomach surgery or diseases like Crohns also might need Vitamin B12 injections

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