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Serious reactions are rare, but they happen when safety protocols slip

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MCF 10A (breast epithelial, ATCC CRL-10317) were cultured in high glucose Dulbeccos modified Eagles (DMEM) medium and HAMS F12 (1:1 ratio, Euroclone), supplemented with 5% horse serum (Gibco), 1% L-glutamine (Gibco), 1% penicillin/streptomycin (Gibco), 10 g/ml insulin (Sigma), 0.5 g/ml hydrocortisone (Sigma), and 20 ng/ml EGF (Cell Guidance Systems)

10.1016/j.placenta.2016.12.005 Placenta 71 GrayM