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A monoclonal antibody to -SMA and caspase-3 was used to incubate tissue sections, and the expression of these proteins was detected using the chromogen 3,3-diaminobenzidine tetrahydrochloride (DAB, Sigma-Aldrich ) stain
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Subsequent rises in IGF-1 concentrations were noted, persisting for one to two weeks, with prolonged activity observed in repeated exposures
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It contains 0.9% benzyl alcohol, which acts as a preservative to inhibit the growth of bacteria
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