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The annealed forward and reverse oligonucleotides were ligated into the pAAV-U6-ZsGreen1 vector (Takara Bio, Japan)

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Seek immediate emergency medical attention if you have symptoms such as a sudden severe headache, confusion, seizure, trouble with speech or vision, numbness or weakness, fainting, personality change, fever, a stiff neck, or a headache after exercise or after a head injury, Dr