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IHC After incubation with secondary antibody, slides were washed in PBS and incubated in SignalStain DAB (CST, 8059) for 5 min or until the horseradish peroxidase signal was visible and the reaction stopped in dH 2 O

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Structural Differences From Native IGF-1 The LR3 designation refers to two specific modifications to the native IGF-1 sequence