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Blots were blocked in either 5% (w/v) nonfat milk (Lab Scientific, M-0841) or 1% (w/v) BSA in 1 Tris-buffered saline with Tween (TBST) (Teknova, T1688) for 3060 min at room temperature, incubated with primary antibodies in TBST for 1 h at room temperature, washed three times with TBST for 5 min each, incubated with fluorophore-conjugated secondary antibodies for 30 min at room temperature, washed three times with TBST and imaged on an Odyssey CLx imager (LI-COR) or ChemiDoc XRS+ imager with Clarity western enhanced chemiluminescence blotting substrates (Bio-Rad)

Medications and Glucose-6-Phosphate Dehydrogenase Deficiency An Evidence-Based Review Ilan Youngster Lidia Arcavi Renata Schechmaster Yulia Akayzen Hen Popliski Janna Shimonov Svetlana Beig Matitiahu Berkovitch Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect and one of the most common genetic disorders worldwide, with an estimated 400 million people worldwide carrying a mutation in the G6PD gene that causes deficiency of the enzyme

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GAPDH and PKM2 are two major enzymes in glycolysis that regulated by sirtuins

Oral Supplements For those with dietary deficiency and normal absorption, oral B12 supplements can be effective