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After 96 h, wells were aspirated, washed with PBS, and a fixative/staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399) was added

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Setbacks are normal

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If someone skips protein at breakfast, has a light lunch, and then tries to eat 80 grams at dinner, it can lead to feelings of nausea and failure