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Animal experiments were carried out in accordance with the animal welfare guidelines of Akita University and the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines

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The total number of the cells was evaluated based on the following formula: where Q was the total number of sexual lineage cells observed in all disectors, h was the optical disector's height, a/f was the area of the counting frame, P was the total number of observed frames, BA was the setting of the microtome, and t was the mean of the final section thickness [36]

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This detoxification process primarily occurs in the liver, where GSH binds to foreign compounds