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75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)
Qin, C., Yang, S., Chu, Y
This process is called oxidative stress, and it's one of the primary drivers of visible skin aging
The retro-inverso D-amino acid configuration of FOXO4-DRI confers resistance to proteolytic enzymes, a pharmacokinetic property critical for maintaining peptide stability in complex biological research environments
PMID: 32690803