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We collected single particle datasets from the purified DDD-Ube2e2-COP1-c-Jun sample (Supplementary Table 3)

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For elution, 14 L nuclease-free water was added to the dried beads, mixed thoroughly, incubated at room temperature for 10 min before collecting the supernatant

Once we have a clear picture of root causes whether its SIBO, candida, parasites, food sensitivities, or a combination we build a layered protocol: remove the offending agents, support digestion and detoxification, and then deploy targeted peptides to accelerate mucosal repair

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