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Immunohistochemical analysis also revealed a significant decrease in the hepatic expression of both proapoptotic markers Bax and caspase-3 with significant increase in antiapoptotic Bcl-2 expression in rats treated with NTZ-200, NAC or 4-PBA when compared to MTX group (Figure 9)

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c, Protein expression (western blot) of GPX4, ACLS4, and LPCAT3 in HK-2 cells treated with 20 ng/mL TGF- for 48 h with or without GPX4 overexpression for 48 h ( n =3)

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Potential Benefits of Combining Berberine and L-Carnitine Whilst there is limited direct research on the combined effects of berberine and L-carnitine, examining their individual mechanisms suggests potential complementary actions, particularly in metabolic health and energy metabolism