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the achilles tendons were harvested by aseptic procedures followed by dissection into pieces at size about 1.5 to 2.0 mm^3 and separation into six-well culture plates
Mutations in DDHD2, encoding an intracellular phospholipase A1, cause a recessive form of complex hereditary spastic paraplegia
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It is possible to inject subcutaneously into the fatty tissue of the abdomen, thigh, or upper arm
Timing: Inject at a consistent time each day and rotate injection sites systematically
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