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The isolated cells were cultured for 1 hour in DMEM containing 1.0 g/L D-glucose, 10% FBS, 100 U/mL penicillin and 0.1 mg/mL streptomycin

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Culture and transient transfection of 293T cells 293T cells were routinely cultured in DMEM (Sigma) supplemented with 10% fetal bovine serum and penicillin and streptomycin

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